Agenda items

27 - 29 Nov 2017. This course provides you with Capillary Electrophoresis (CE) fundamentals and operating modes, as well as practical tips to obtain fit-for-purpose methods. The method validation focuses on the ICH Q2 guideline, with many real-life examples from the pharmaceutical and biotech industry. Troubleshooting combines fundamental understanding with pragmatic problem solving and prevention. Although the focus is on (bio)pharmaceuticals, the principles apply for most CE methods.

See here for the 2017 brochure.

Learning outcome

After this training, you will understand the fundamentals and be able to couple method development and validation to the purpose of your analysis. You will be better able to develop robust, precise and sensitive methods and will have a better understanding about the critical parameters, Quality by Design and good working practice of CE. You will have a good understanding what the validation parameters are and you will be able to make a to-the-point validation protocol. Your gain in knowledge will reduce the amount of troubleshooting needed in daily practice, but if needed, you’ll have a good understanding of where to start and what to look for.

Course content

  • The separation mechanism
  • Different modes of CE, including CZE, CGE and cIEF
  • CE method development
  • CE instrumental aspects and Good Working Practices
  • Quality by Design and Design of Experiments
  • Method validation purpose and phase-related approach
  • ICH Q2 guideline for analytical method validation
  • Troubleshooting strategy
  • Preventing the need for troubleshooting

Feedback from previous courses.

Registration

The 2017 course takes places 27 - 29 November 2017 in Naarden-Bussum, close to Amsterdam. Early bird reduced fees until 15 July 2017. Deadline for registration 15 October 2017. After 15 October, only after contact with Kantisto and based on available space. Minumum attendance 5 people, maximum 15 people. Refund policy: Written cancellation before 1 Aug 2017 will result in a full refund minus 20 % processing fee. Cancellations made after 1 Aug 2017 are not refundable, but registration can be transfered to another person. More details in the brochure. Course fee includes three hotel nights and breakfast, lunches, and course hand-outs.

Kantisto also offers on-site courses with content tailored to your needs.

Please send an e-mail or use Kantisto's contact form.

Good CE Working Practice is discussed in this course, illustrated with many real-life examples of small molecules and proteins. Although the focus is on pharmaceuticals, the principles apply for most quantitative CE methods.

This course starts off with a refreshment of the fundamentals and operating modes of capillary electrophoresis. The approaches to method optimization during CE method development will be described, zooming in to the influence of key operating parameters and good working practice. Many practical examples and applications from the  pharmaceutical and biotechnology industry will be discussed. After this training you will be able to couple method development and validation to the purpose of your analysis. You will have a good understanding what the validation parameters are and you will be able to make a to-the-point validation protocol. This is based on a better understanding what the critical method parameters are and increased knowledge of good working practices of CE. As a consequence the amount of trouble shooting needed in daily practice will be reduced, but if you still do need to troubleshoot, you will have a good understanding of where to start and what to look for.

Training tailor-made, on-site, on-the-job - please contact Kantisto.

Quality by Design for analytical methods: why would you need QbD for analysis? What tools are available and how do you use them? How to set up an experimental design? What are the prerequisites for successful pharmaceutical analysis methods? What are pharmaceutical methods for anyhow? What is the impact of simple sample preparations steps such as pipetting and dilution? Is there a way of working that will keep the variation low and precision good? The course includes many practical examples.

Training on-site, on-the-job, if requested. For more information, costs, registration and planning,  please contact Kantisto.

This is a practical on-site training on the daily use and work flow with ChemStation software for Agilent 1600 or 7100 Capillary Electrophoresis instruments.

Please contact Kantisto for further information.

The content of this training will be fine-tuned in consultation with the participants. The fundamentals on electrophoresis and electro-osmosis theory and different CE modes are refreshed and attention is paid to Good working practice on CE, the troubleshooting process, preventing troubleshooting and case studies. Training on-site, on-the-job, if requested.

For more information, costs, registration and planning,  please contact Kantisto.